Yes, Tsingke offers free codon optimization.
Codon optimization enhances gene expression by using preferred codons and avoiding rare ones for a specified host. This optimization correlates strongly with improved gene expression levels. Codelifex's unique GeneOptimizer software supports optimization for tens of thousands of hosts and offers customized services. Key optimization parameters include codon preference, GC content, restriction enzyme sites (deletion), poly structures, and sequence repeats.
Yes, you can use your own vector. It is recommended to ship your vector in advance; otherwise, the turnaround time may be extended by 3 business days to verify the vector.
We need you to ship at least 2 μg of the plasmid and provide the full sequence information of the vector. If the full sequence is not available, please provide at least 200 bp upstream and downstream of the cloning site, along with the vector's antibiotic resistance information.